Eco-city has become an inevitable product of sustainable development of the world's cities. Based on the eco-city concept and the index system proposed at home and abroad, we inherit the basic ideas and principles of the relevant theoretical concepts of ecological cities at home and abroad. Starting from the ecological and ecological city development model, we believe that ecological cities are based on the concept of ecological civilization to establish a coordinated and sustainable human habitat for economic, social and natural development. We explores further, and establishes an index system based on three subsystems: living and working in peace, resources and environment, and development potential. Through factor analysis methods, it is applicable to city-level cities in the middle reaches of the Yangtze River. The 28 eco-city developments were ranked for evaluation. According to the scores and development status of the factor analysis model of each ecological city, the development level of the ecological city in the middle reaches of the Yangtze River is divided into three stages: developed ecological city, developing ecological city, and backward ecological city. The ecological cities in the prefecture-level cities in the middle reaches of the Yangtze River include 10 developed ecological cities such as Wuhan, Nanchang, Changsha, Yingtan, Jingdezhen, Xinyu, Ezhou, Xiangtan, Huangshi, Pingxiang; 6 developing ecological city such as Yichun, Zhuzhou, Jingmen, Jiujiang, Loudi, Changde; 12 backward ecological cities such as Fuyang, Fuzhou, Jingzhou, Huanggang, Yiyang, Yueyang, Xiaogan, Ji'an, Shangrao, Hengyang, Changde, Xianning. The proportion of developed and developing ecological cities in the middle reaches of the Yangtze River has reached 57.14%. Among them, the level of ecological cities in Jiangxi Province is higher than those in Hubei and Hunan.