Lignin is the second most abundant biopolymer on earth, and a kind of renewable resource in nature. It plays important roles in biofuel production, as it could form non-productive adsorption with cellulase. Due to the structural complexity of lignin, limited result was obtained so far. The dehydrogenation polymer (DHP) was usually used to study the features of lignin, whose structure was simpler than that of lignin. In this study, new method was employed to synthesise coniferin. The yield based on the starting reactant was 51.44%, which was 19.67% higher than the traditional method. Furthermore, Guaiacyl-type lignin polymer models (DHP) were prepared from coniferin by Zutropfverfahren (ZT) and Zulaufverfahren (ZL) with ß-glucosidase, peroxidase and glucose oxidase. The DHPs were characterised by GPC, FT-IR, and HSQC. The molecular weights of ZT and ZL DHP were 2032 and 2332 g/mol. FT-IR analysis and Semi-quantitative analysis by 2D HSQC NMR indicated that typical lignin structures such as β-5, β-O-4 and β-β existed in DHP and ZT DHP had higher content of β-β and C-H than ZL DHP. Finally, the DHP were added to Avicel to determine its effect on glucose release. The results showed that in the presence of ZT and ZL DHP, the cellulose conversion of avicel are 26.35% and 29.32% when the cellulase loading was 50 FPU/g cellulose, while the cellulose conversion of avicel is 37.16% without DHP. It is found that molecular weight and PDI of DHPs were negatively correlated with cellulase hydrolysis of Avicel. And DHP with higher content of β-β and C-H had great effect on cellulase hydrolysis of Avicel. The results could broaden horizon of lignin-cellulase complex and decrease the cost of biofuel production accordingly.