A significant percentage of energy produced is used for cooling systems. For this reason, improvements in cooling systems can provide significant energy savings. In this study, a comparative result of the energy and exergy changes that occur in the system when different working fluids were used in the ejector cooling system with a unique design is presented. The system comprised different components such as an ejector, a steam generator, an evaporator, an air condenser, and a pump. This study aimed to determine the parameters that affect the thermodynamic performance of different working fluids. As a result, ideal working conditions were found by calculating the working fluid with the highest energy and exergy efficiency.