Thermoelectric coolers (TECs) are becoming the focus of attention of researchers on a global scale due to their various advantages such as quiet and vibration-free operation and the absence of moving parts, as well as being energy efficient and compact. It is frequently preferred especially for portable cooler applications. This study is about the design, fabrication, and thermodynamic analysis of a solar-assisted nanofluid integrated thermoelectric vaccine cabinet. It is aimed to investigate the effects of using nanofluids as refrigerants in portable thermoelectric vaccine cabinet applications on basic performance parameters. For this purpose, it is planned to design and manufacture a cooling cabinet. Then, the Peltier assembly, with a water-cooled block integrated on its hot surface, will be placed from the cooling cabinet, and a water-to-air heat exchanger will be used to transfer the heat taken from the hot surface of the Peltier to the environment with the help of the refrigerant. Hybrid nanofluids will be used as refrigerants in the system. PV panels will be placed on the upper surface of the cooling cabinet in order to benefit from solar energy. In order to store the electrical power obtained from the PV panel, a battery will be located at the bottom of the cabinet. Cooled cabin temperatures will be observed with experimental studies, and the results will be compared with the situation where no nanofluid is used as the reference situation.